Pleural Fluid Analysis
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Thoracentesis fluid analysis.
To assess and categorize fluid obtained from within the pleural space for infection, cancer, and blood as well as identify the cause of its accumulation.
Pleural fluid collected in a green-top (heparin) tube for amylase, cholesterol, glucose, lactate dehydrogenase (LDH), pH, protein, and triglycerides; lavender-top (EDTA) tube for cell count; sterile containers for microbiology specimens; of fluid in a clear container with anticoagulant for cytology. Ensure that there is an equal amount of fixative and fluid in the container for cytology.
(Method: Spectrophotometry for amylase, cholesterol, glucose, LDH, protein, and triglycerides; ion-selective electrode for pH; automated or manual cell count; macroscopic and microscopic examination of cultured microorganisms; microscopic examination of specimen for microbiology and cytology)
|Amylase||Parallels serum values|
|Cholesterol||Parallels serum values|
|CEA||Parallels serum values|
|Glucose||Parallels serum values|
|LDH||Less than 200 units/L|
|Fluid LDH-to-serum LDH ratio||0.6 or less|
|Fluid protein-to-serum protein ratio||0.5 or less|
|Triglycerides||Parallel serum values|
|RBC count||None seen|
|WBC count||Less than 1,000 cells/microL|
|Gram stain||No organisms seen|
|Cytology||No abnormal cells seen|
|CEA = carcinoembryonic antigen; LDH = lactate dehydrogenase; RBC = red blood cell; WBC = white blood cell.|